We are seeking a Postdoctoral Research Associate to investigate replication fork fusion events. You will use Escherichia coli as a model system to uncover fundamental aspects of this crucial final stage of genome duplication.
All organisms need to replicate their chromosomes with high fidelity to ensure that genetic information passed on to the next generation is accurate. Chromosome duplication initiates at defined origins and replication forks then proceed in opposite directions. DNA replication terminates when a replication fork meets the end of a chromosome or another fork travelling in the opposite direction. It is becoming apparent that incorrectly processed fork fusion events result in problems for genome stability. We have established a system to investigate E. coli fork fusion events in vitro. You will build on this to characterise the DNA intermediates and protein dynamics at fusing forks. These analyses will form a key part of an interdisciplinary collaboration with Dr. Christian Rudolph at Brunel University. This project will combine biochemistry, genetics and live cell imaging to understand replication fork fusion and how errors in this process can result in pathologies.
The main responsibilities are:
You will have a relevant PhD and strong skills in molecular biology and/or biochemistry. Experience with protein purification and a background in DNA replication will be beneficial.
You will have knowledge of a range of research techniques and methodologies and research expertise in an area that will complement and enhance the department’s research strategy and goals. You will have experience of writing up research work for publication and have the ability to work as part of a team and also to work independently using your own initiative. You will also have the competency to make presentations at conferences or exhibit work in other appropriate events.
The post is full-time and available for up to 18 months starting from April 2018 onwards. The starting salary will be within the range £31,604 - £38,832 per annum, depending on relevant experience.
Closing date: 20 March 2018
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